Wednesday, July 2, 2014

The Best Discovery In Diagnosis; Elisa Test Kits

By Sally Delacruz


Recently, scientists discovered a new method of diagnosing illnesses, antigens, antibodies and other foreign materials. Scientists normally mix reagents with samples of biological materials to be tested, and then wait for color change. Elisa test kits carry the tools used in carrying out tests for the existence of antigens and antibodies.

These kits are always made with the purpose of providing accuracy and consistency in research results. They are therefore required to be sensitive, easy to follow, precise and specific to individual tests. This can be easily achieved through rigorous testing. The testing may involve validating with many samples from, serum, saliva, feces and urine.

The main reason for manufacturing these kits is enhancement of consistency and accuracy by way of promoting visibility. Devices used in testing, therefore, have to be easy to understand and sensitive to changes in the reagents. They also have to be specific to certain tests and must also be precise. Rigorous testing is a very important exercise in ensuring that the kits satisfy all these requirements.

These kits help in making research faster. A device that is deficient of the ability to reproduce results and sensitivity in making readings during research is not the best for Elisa test. Researchers might end up using a lot of money and time as a result of using these apparatus. This means that one needs to take a lot of caution while buying them from any company.

Besides antibodies, many other factors are crucial during the manufacture of these tools. For instance, rigorous antibody screening must be done, good standards chosen, steps for sample preparation developed and essays properly validated with the help of matrices. All these process are done with the purpose of attaining effectiveness.

The kits are capable of detecting items that are present in low concentrations for the sake of diagnosing and treating a number of diseases during their early stages. It has, previously been used in diagnosing different types of illnesses in both animals and plants. This technology has also been used for ensuring quality control in many industries.

This process involves putting antigens onto a surface, and then applying specific antibodies onto them. Specific substrates are then added to this combination of antibodies and antigens. Results are always in form of color change.

When the kits are used in carrying out the same test, the tools on the kit are dipped into a test tube of samples collected. The set-up is then washed and then incubated in chromogens. The kit itself is made up of rods with about twelve ogives that protrude. The process of washing and incubating normally occurs when the ogive is immersed in small wells made of micro-plates containing reagents.

This kit is compatible with different types of reagents. Because of this quality, simultaneous tests for different antigens can be carried out. For this to be possible, the Ogive is sensitized to react to different reagents. The sensitivity of the tools can also be improved through raising the amount of samples.

The ability of these testing tools to establish the quantity of antibodies has been applied in testing for HIV and other immune deficiency related diseases. When few antibodies are observed in the body, then the victim is declared HIV positive. The fact that they are portable also contributes heavily towards making Elisa kits popular. Care has to be taken while buying them; most manufacturers do not pay attention to quality.




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